PrimePCR™ Probe Assay: TNFSF12, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TNFSF12, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TNFSF12, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0056014
Assay Design:   exonic
Chromosome Location:   17:7460837-7461011question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000293825

Gene Information

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF but displays a much wider tissue distribution. This cytokine which exists in both membrane-bound and secreted forms can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977 TNFSF12-TNFSF13. [provided by RefSeq Oct 2010]

Gene Symbol:   TNFSF12
Gene Name:   tumor necrosis factor (ligand) superfamily, member 12
Aliases:   APO3L, DR3LG, MGC129581, MGC20669, TWEAK
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000239697
Entrez:   8742
Chromosome Mapping:   17p13

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