PrimePCR™ Probe Assay: MAP3K1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAP3K1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAP3K1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026621
Assay Design:   Intron-spanning
Chromosome Location:   5:56184128-56189461question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000399503

Gene Information

The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq Mar 2012]

Gene Symbol:   MAP3K1
Gene Name:   mitogen-activated protein kinase kinase kinase 1
Aliases:   MAPKKK1, MEKK, MEKK1
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000095015
Entrez:   4214
Chromosome Mapping:   5q11.2

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