PrimePCR™ Probe Assay: LIMK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LIMK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LIMK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028201
Assay Design:   Intron-spanning
Chromosome Location:   7:73511494-73513445question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000418310 ENST00000336180 ENST00000423685 ENST00000419043 ENST00000538333

Gene Information

There are approximately 40 known eukaryotic LIM proteins so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq Feb 2011]

Gene Symbol:   LIMK1
Gene Name:   LIM domain kinase 1
Aliases:   LIMK, LIMK-1
RefSeq:   NC_000007.13 NG_008129.1 NT_007933.15
Ensembl:   ENSG00000106683
Entrez:   3984
Chromosome Mapping:   7q11.23

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