PrimePCR™ Probe Assay: MDH2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MDH2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MDH2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028953
Assay Design:   Intron-spanning
Chromosome Location:   7:75694192-75695747question
Amplicon Length:   201
Splice Variants Targeted:   ENST00000315758 ENST00000443006 ENST00000432020

Gene Information

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. [provided by RefSeq Jul 2008]

Gene Symbol:   MDH2
Gene Name:   malate dehydrogenase 2, NAD (mitochondrial)
Aliases:   M-MDH, MDH, MGC:3559, MOR1
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000146701
Entrez:   4191
Chromosome Mapping:   7cen-q22

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