PrimePCR™ Probe Assay: MYH3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MYH3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MYH3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029821
Assay Design:   Intron-spanning
Chromosome Location:   17:10555803-10558224question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000226209

Gene Information

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes Freeman-Sheldon syndrome and Sheldon-Hall syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   MYH3
Gene Name:   myosin, heavy chain 3, skeletal muscle, embryonic
Aliases:   HEMHC, MYHC-EMB, MYHSE1, SMHCE
RefSeq:   NC_000017.10 NG_011537.1 NT_010718.16
Ensembl:   ENSG00000109063
Entrez:   4621
Chromosome Mapping:   17p13.1

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