PrimePCR™ Probe Assay: FGD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FGD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FGD1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030301
Assay Design:   Intron-spanning
Chromosome Location:   X:54492221-54494286question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000375135

Gene Information

This gene encodes a protein that contains Dbl (DH) and pleckstrin (PH) homology domains and is similar to the Rho family of small GTP-binding proteins. The encoded protein specifically binds to the Rho family GTPase Cdc42Hs and can stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. Defects in this gene are the cause of faciogenital dysplasia and X-linked mental retardation syndromatic 16.[provided by RefSeq Mar 2011]

Gene Symbol:   FGD1
Gene Name:   FYVE, RhoGEF and PH domain containing 1
Aliases:   AAS, FGDY, MRXS16, ZFYVE3
RefSeq:   NC_000023.10 NG_008054.1 NT_011630.14
Ensembl:   ENSG00000102302
Entrez:   2245
Chromosome Mapping:   Xp11.21

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