PrimePCR™ Probe Assay: CD27, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CD27, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CD27, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031070
Assay Design:   Intron-spanning
Chromosome Location:   12:6554630-6559385question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000266557

Gene Information

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70 and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA) a proapoptotic protein can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq Jul 2008]

Gene Symbol:   CD27
Gene Name:   CD27 molecule
Aliases:   MGC20393, S152, T14, TNFRSF7, Tp55
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000139193
Entrez:   939
Chromosome Mapping:   12p13

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