PrimePCR™ Probe Assay: SAG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SAG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SAG, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031587
Assay Design:   Intron-spanning
Chromosome Location:   2:234224725-234227433question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000447536 ENST00000409110 ENST00000415974 ENST00000252857 ENST00000478615

Gene Information

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones neurotransmitters or sensory signals. S-arrestin also known as S-antigen is a major soluble photoreceptor protein that is involved in desensitization of the photoactivated transduction cascade. It is expressed in the retina and the pineal gland and inhibits coupling of rhodopsin to transducin in vitro. Additionally S-arrestin is highly antigenic and is capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene have been associated with Oguchi disease a rare autosomal recessive form of night blindness. [provided by RefSeq Jul 2008]

Gene Symbol:   SAG
Gene Name:   S-antigen; retina and pineal gland (arrestin)
Aliases:   DKFZp686D1084, DKFZp686I1383, RP47, S-AG
RefSeq:   NC_000002.11 NG_009116.1 NT_005120.16
Ensembl:   ENSG00000130561
Entrez:   6295
Chromosome Mapping:   2q37.1

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