PrimePCR™ Probe Assay: POLR1D, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: POLR1D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POLR1D, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040967
Assay Design:   exonic
Chromosome Location:   13:28196024-28196135question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000380948 ENST00000286096 ENST00000302979 ENST00000399697 ENST00000562377 ENST00000296487 ENST00000268711 ENST00000536708 ENST00000338399 ENST00000260947 ENST00000449967 ENST00000375664 ENST00000375646 ENST00000347934 ENST00000354911 ENST00000220676

Gene Information

The protein encoded by this gene is a component of the RNA polymerase I and RNA polymerase III complexes which function in the synthesis of ribosomal RNA precursors and small RNAs respectively. Mutations in this gene are a cause of Treacher Collins syndrome (TCS) a craniofacial development disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq Apr 2011]

Gene Symbol:   POLR1D
Gene Name:   polymerase (RNA) I polypeptide D, 16kDa
Aliases:   AC19, FLJ20616, MGC9850, POLR1C, RPA16, RPA9, RPAC2, RPC16, RPO1-3, TCS2
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000186184
Entrez:   51082
Chromosome Mapping:   13q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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