PrimePCR™ Probe Assay: GSTM2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GSTM2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GSTM2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041083
Assay Design:   exonic
Chromosome Location:   1:110214104-110217372question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000369836 ENST00000326729 ENST00000369833 ENST00000336075 ENST00000369831 ENST00000460717 ENST00000241337 ENST00000414179 ENST00000369829 ENST00000442650 ENST00000309851 ENST00000369823 ENST00000256593 ENST00000369813 ENST00000369812 ENST00000549687 ENST00000548392 ENST00000360820

Gene Information

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha kappa mu omega pi sigma theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds including carcinogens therapeutic drugs environmental toxins and products of oxidative stress by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. [provided by RefSeq Jul 2008]

Gene Symbol:   GSTM2
Gene Name:   glutathione S-transferase mu 2 (muscle)
Aliases:   GST4, GSTM, GSTM2-2, GTHMUS, MGC117303
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000213366
Entrez:   2946
Chromosome Mapping:   1p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.170000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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