PrimePCR™ Probe Assay: CCL3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CCL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CCL3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041355
Assay Design:   exonic
Chromosome Location:   17:34415896-34415987question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000442488 ENST00000427185 ENST00000331178 ENST00000226091 ENST00000261769 ENST00000422202 ENST00000429149 ENST00000336798 ENST00000394329 ENST00000225245 ENST00000378354 ENST00000379388 ENST00000422211 ENST00000272252 ENST00000225396 ENST00000566881 ENST00000406386 ENST00000565629

Gene Information

This locus represents a small inducible cytokine. The encoded protein also known as macrophage inflammatory protein 1 alpha plays a role in inflammatory responses through binding to the receptors CCR1 CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq Sep 2010]

Gene Symbol:   CCL3
Gene Name:   chemokine (C-C motif) ligand 3
Aliases:   G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3
RefSeq:   NC_000017.10 NG_027730.1 NT_010799.15
Ensembl:   ENSG00000006075
Entrez:   6348
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.800000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download