PrimePCR™ Probe Assay: NUP62, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NUP62, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NUP62, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041526
Assay Design:   exonic
Chromosome Location:   19:50413058-50430962question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000413454 ENST00000596680 ENST00000600935 ENST00000599788 ENST00000594673 ENST00000596437 ENST00000600645 ENST00000599567 ENST00000595761 ENST00000593652 ENST00000596217 ENST00000597723 ENST00000597029 ENST00000352066 ENST00000422090 ENST00000356401 ENST00000449376 ENST00000409600

Gene Information

The nuclear pore complex is a massive structure that extends across the nuclear envelope forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq Jul 2008]

Gene Symbol:   NUP62
Gene Name:   nucleoporin 62kDa
Aliases:   DKFZp547L134, FLJ20822, FLJ43869, IBSN, MGC841, SNDI, p62
RefSeq:   NC_000019.9 NG_021170.1 NT_011109.16 NG_023448.1
Ensembl:   ENSG00000213024
Entrez:   23636
Chromosome Mapping:   19q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 36.140000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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