PrimePCR™ Probe Assay: DIABLO, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DIABLO, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DIABLO, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041684
Assay Design:   exonic
Chromosome Location:   12:122701306-122702842question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000353548 ENST00000443649 ENST00000464942 ENST00000267169 ENST00000439489 ENST00000541273 ENST00000474004 ENST00000540535 ENST00000541656 ENST00000446652 ENST00000485724 ENST00000413918

Gene Information

This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq Dec 2008]

Gene Symbol:   DIABLO
Gene Name:   diablo, IAP-binding mitochondrial protein
Aliases:   DIABLO-S, FLJ10537, FLJ25049, SMAC, SMAC3
RefSeq:   NC_000012.11 NT_009755.19
Ensembl:   ENSG00000184047
Entrez:   56616
Chromosome Mapping:   12q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.630000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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