PrimePCR™ Probe Assay: MAD1L1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAD1L1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAD1L1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049137
Assay Design:   exonic
Chromosome Location:   7:2262223-2262349question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000409318 ENST00000376840 ENST00000327581 ENST00000406902 ENST00000361034 ENST00000257860 ENST00000505231 ENST00000435205 ENST00000341356 ENST00000393893 ENST00000479991 ENST00000545866 ENST00000402746 ENST00000399654 ENST00000406869 ENST00000445959 ENST00000429625 ENST00000455998 ENST00000429779 ENST00000265854 ENST00000367236 ENST00000166244 ENST00000318083 ENST00000356751 ENST00000594582 ENST00000419611 ENST00000377367 ENST00000366621 ENST00000430629 ENST00000536657 ENST00000567483 ENST

Gene Information

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   MAD1L1
Gene Name:   MAD1 mitotic arrest deficient-like 1 (yeast)
Aliases:   MAD1, PIG9, TP53I9, TXBP181
RefSeq:   NC_000007.13 NT_007819.17 NG_011518.1
Ensembl:   ENSG00000002822
Entrez:   8379
Chromosome Mapping:   7p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.580000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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