PrimePCR™ Probe Assay: ARHGEF7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ARHGEF7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARHGEF7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050196
Assay Design:   exonic
Chromosome Location:   13:111862323-111870116question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000317133 ENST00000449979 ENST00000491775 ENST00000466143 ENST00000218789 ENST00000375736 ENST00000467053 ENST00000426073 ENST00000426768 ENST00000375723 ENST00000370623 ENST00000375741 ENST00000375739 ENST00000375737 ENST00000544132

Gene Information

Rho GTPases play a fundamental role in numerous cellular processes triggered by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. This protein can induce membrane ruffling. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ARHGEF7
Gene Name:   Rho guanine nucleotide exchange factor (GEF) 7
Aliases:   BETA-PIX, COOL-1, COOL1, DKFZp686C12170, DKFZp761K1021, KIAA0142, KIAA0412, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB
RefSeq:   NC_000013.10 NT_009952.14
Ensembl:   ENSG00000102606
Entrez:   8874
Chromosome Mapping:   13q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.960000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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