PrimePCR™ Probe Assay: FOSB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FOSB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FOSB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051359
Assay Design:   exonic
Chromosome Location:   19:45976142-45976290question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000585836 ENST00000417353 ENST00000353609 ENST00000591858 ENST00000443841 ENST00000592436 ENST00000592811 ENST00000586615

Gene Information

The Fos gene family consists of 4 members: FOS FOSB FOSL1 and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family thereby forming the transcription factor complex AP-1. As such the FOS proteins have been implicated as regulators of cell proliferation differentiation and transformation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   FOSB
Gene Name:   FBJ murine osteosarcoma viral oncogene homolog B
Aliases:   AP-1, DKFZp686C0818, G0S3, GOS3, GOSB, MGC42291
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000125740
Entrez:   2354
Chromosome Mapping:   19q13.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.650000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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