PrimePCR™ Probe Assay: PRMT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PRMT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRMT1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051404
Assay Design:   exonic
Chromosome Location:   19:50183747-50183844question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000526224 ENST00000527412 ENST00000529836 ENST00000524771 ENST00000454376 ENST00000391851 ENST00000534465 ENST00000528623 ENST00000527382 ENST00000532489 ENST00000529284 ENST00000374429 ENST00000300737 ENST00000527651 ENST00000533977 ENST00000526596 ENST00000262958 ENST00000361756 ENST00000393713 ENST00000530137 ENST00000545854 ENST00000344094 ENST00000262376 ENST00000590769 ENST00000396658 ENST00000545171 ENST00000272117 ENST00000429204 ENST00000504457 ENST00000505395

Gene Information

This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq Dec 2011]

Gene Symbol:   PRMT1
Gene Name:   protein arginine methyltransferase 1
Aliases:   ANM1, HCP1, HRMT1L2, IR1B4
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000126457
Entrez:   3276
Chromosome Mapping:   19q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.770000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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