PrimePCR™ Probe Assay: CYP2E1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CYP2E1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CYP2E1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051588
Assay Design:   exonic
Chromosome Location:   10:135350644-135350745question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000463117 ENST00000421586 ENST00000418356 ENST00000252945

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol the diabetic state and starvation. The enzyme metabolizes both endogenous substrates such as ethanol acetone and acetal as well as exogenous substrates including benzene carbon tetrachloride ethylene glycol and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates this enzyme may be involved in such varied processes as gluconeogenesis hepatic cirrhosis diabetes and cancer. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP2E1
Gene Name:   cytochrome P450, family 2, subfamily E, polypeptide 1
Aliases:   CPE1, CYP2E, P450-J, P450C2E
RefSeq:   NC_000010.10 NG_008383.1 NT_008818.16
Ensembl:   ENSG00000130649
Entrez:   1571
Chromosome Mapping:   10q24.3-qter

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.720000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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