PrimePCR™ Probe Assay: NCAM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NCAM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NCAM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052707
Assay Design:   exonic
Chromosome Location:   11:113075061-113075200question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000393196 ENST00000533760 ENST00000401611 ENST00000316851 ENST00000309791 ENST00000297848 ENST00000247781 ENST00000591454 ENST00000586162 ENST00000593059 ENST00000591218 ENST00000590031 ENST00000576604 ENST00000592855 ENST00000586493 ENST00000589067 ENST00000442738 ENST00000407936 ENST00000405557

Gene Information

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jun 2011]

Gene Symbol:   NCAM1
Gene Name:   neural cell adhesion molecule 1
Aliases:   CD56, MSK39, NCAM
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000149294
Entrez:   4684
Chromosome Mapping:   11q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.930000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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