PrimePCR™ Probe Assay: PTPRR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PTPRR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PTPRR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052858
Assay Design:   exonic
Chromosome Location:   12:71094931-71095073question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000440835 ENST00000283228 ENST00000342084 ENST00000549308 ENST00000550661 ENST00000378778

Gene Information

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. This PTP possesses an extracellular region a single transmembrane region and a single intracellular catalytic domain and thus represents a receptor-type PTP. Silencing of this gene has been associated with colorectal cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares a symbol (PTPRQ) with another gene protein tyrosine phosphatase receptor type Q (GeneID 374462) which is also located on chromosome 12. [provided by RefSeq May 2011]

Gene Symbol:   PTPRR
Gene Name:   protein tyrosine phosphatase, receptor type, R
Aliases:   DKFZp781C1038, EC-PTP, FLJ34328, MGC131968, MGC148170, PCPTP1, PTP-SL, PTPBR7, PTPRQ
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000153233
Entrez:   5801
Chromosome Mapping:   12q15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.920000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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