PrimePCR™ Probe Assay: CXCL13, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CXCL13, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CXCL13, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052976
Assay Design:   exonic
Chromosome Location:   4:78532784-78532886question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000286758

Gene Information

B lymphocyte chemoattractant independently cloned and named Angie is a CXC chemokine strongly expressed in the follicles of the spleen lymph nodes and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages) apparently by stimulating calcium influx into and chemotaxis of cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq Jul 2008]

Gene Symbol:   CXCL13
Gene Name:   chemokine (C-X-C motif) ligand 13
Aliases:   ANGIE, ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000156234
Entrez:   10563
Chromosome Mapping:   4q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995700
y-intercept 35.340000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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