PrimePCR™ Probe Assay: ROR2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ROR2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ROR2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053867
Assay Design:   exonic
Chromosome Location:   9:94486813-94486936question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000375715 ENST00000375708

Gene Information

The protein encoded by this gene is a receptor protein tyrosine kinase and type I transmembrane protein that belongs to the ROR subfamily of cell surface receptors. The protein may be involved in the early formation of the chondrocytes and may be required for cartilage and growth plate development. Mutations in this gene can cause brachydactyly type B a skeletal disorder characterized by hypoplasia/aplasia of distal phalanges and nails. In addition mutations in this gene can cause the autosomal recessive form of Robinow syndrome which is characterized by skeletal dysplasia with generalized limb bone shortening segmental defects of the spine brachydactyly and a dysmorphic facial appearance. [provided by RefSeq Jul 2008]

Gene Symbol:   ROR2
Gene Name:   receptor tyrosine kinase-like orphan receptor 2
Aliases:   BDB, BDB1, MGC163394, NTRKR2
RefSeq:   NC_000009.11 NG_008089.1 NT_008470.19
Ensembl:   ENSG00000169071
Entrez:   4920
Chromosome Mapping:   9q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.740000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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