PrimePCR™ Probe Assay: NKX2-5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NKX2-5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NKX2-5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054834
Assay Design:   exonic
Chromosome Location:   5:172661789-172661882question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000551577 ENST00000329198 ENST00000424406 ENST00000521848 ENST00000517440

Gene Information

This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect and also tetralogy of Fallot which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5 a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2009]

Gene Symbol:   NKX2-5
Gene Name:   NK2 transcription factor related, locus 5 (Drosophila)
Aliases:   CHNG5, CSX, CSX1, FLJ52202, FLJ97166, FLJ97195, FLJ97197, FLJ99536, NKX2.5, NKX2E, NKX4-1
RefSeq:   NC_000005.9 NT_023133.13 NG_013340.1
Ensembl:   ENSG00000183072
Entrez:   1482
Chromosome Mapping:   5q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.980000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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