PrimePCR™ Probe Assay: KRT5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KRT5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KRT5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055058
Assay Design:   exonic
Chromosome Location:   12:52912933-52913608question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000293379 ENST00000399448 ENST00000318974 ENST00000447931 ENST00000456013 ENST00000252245 ENST00000252252 ENST00000252250 ENST00000330722 ENST00000252242 ENST00000549420 ENST00000551275 ENST00000537672 ENST00000354310 ENST00000549979 ENST00000293745 ENST00000398066 ENST00000595832 ENST00000599028 ENST00000595623 ENST00000594686

Gene Information

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq Jul 2008]

Gene Symbol:   KRT5
Gene Name:   keratin 5
Aliases:   CK5, DDD, EBS2, K5, KRT5A
RefSeq:   NC_000012.11 NG_008297.1 NT_029419.12
Ensembl:   ENSG00000186081
Entrez:   3852
Chromosome Mapping:   12q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 37.710000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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