PrimePCR™ Probe Assay: KRT16, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KRT16, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KRT16, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055126
Assay Design:   exonic
Chromosome Location:   17:39767337-39767479question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000301653 ENST00000593067 ENST00000357086 ENST00000456211 ENST00000443694 ENST00000302640 ENST00000354582 ENST00000423119 ENST00000314641 ENST00000355739 ENST00000535557 ENST00000504087 ENST00000515641 ENST00000382582 ENST00000585201 ENST00000397875 ENST00000397866 ENST00000397865 ENST00000359645 ENST00000528160 ENST00000355994 ENST00000269576 ENST00000562889 ENST00000283426

Gene Information

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues including esophagus tongue and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq Jul 2008]

Gene Symbol:   KRT16
Gene Name:   keratin 16
Aliases:   CK16, FNEPPK, K16, K1CP, KRT16A, NEPPK
RefSeq:   NC_000017.10 NG_008301.1 NT_010783.15
Ensembl:   ENSG00000186832
Entrez:   3868
Chromosome Mapping:   17q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.610000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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