PrimePCR™ Probe Assay: HLA-G, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HLA-G, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HLA-G, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055720
Assay Design:   exonic
Chromosome Location:   6:29798571-29798706question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000376828 ENST00000360323 ENST00000376818 ENST00000376815 ENST00000428701 ENST00000420559 ENST00000423373 ENST00000452577 ENST00000457132 ENST00000546634 ENST00000450984 ENST00000449127 ENST00000412263 ENST00000415687 ENST00000547931 ENST00000400665 ENST00000444098 ENST00000445373 ENST00000452715 ENST00000546545 ENST00000400682 ENST00000383621 ENST00000383623 ENST00000383622 ENST00000553052 ENST00000422371 ENST00000428952 ENST00000426863 ENST00000429890 ENST00000550897 ENST00000434881 ENST

Gene Information

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide exons 2 and 3 encode the alpha1 and alpha2 domain which both bind the peptide exon 4 encodes the alpha3 domain exon 5 encodes the transmembrane region and exon 6 encodes the cytoplasmic tail. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-G
Gene Name:   major histocompatibility complex, class I, G
Aliases:   MHC-G
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204632
Entrez:   3135
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.370000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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