PrimePCR™ Probe Assay: POLB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: POLB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POLB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057881
Assay Design:   exonic
Chromosome Location:   8:42229109-42229231question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000265421 ENST00000521290 ENST00000518579 ENST00000517393 ENST00000521492 ENST00000538005

Gene Information

The protein encoded by this gene is a DNA polymerase involved in base excision and repair also called gap-filling DNA synthesis. The encoded protein acting as a monomer is normally found in the cytoplasm but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist but the full-length nature of only one has been described to date. [provided by RefSeq Sep 2011]

Gene Symbol:   POLB
Gene Name:   polymerase (DNA directed), beta
Aliases:   MGC125976
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000070501
Entrez:   5423
Chromosome Mapping:   8p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.050000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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