PrimePCR™ Probe Assay: ITGAV, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ITGAV, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ITGAV, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026077
Assay Design:   Intron-spanning
Chromosome Location:   2:187503136-187506162question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000261023 ENST00000374907 ENST00000433736 ENST00000544640

Gene Information

ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1356 and 8; 'ITGB1' 'ITGB3' 'ITGB5' 'ITGB6' and 'ITGB8') each can interact with extracellular matrix ligands; the alpha V beta 3 integrin perhaps the most studied of these is referred to as the Vitronectin receptor (VNR). In addition to adhesion many integrins are known to facilitate signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq Feb 2009]

Gene Symbol:   ITGAV
Gene Name:   integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
Aliases:   CD51, DKFZp686A08142, MSK8, VNRA
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000138448
Entrez:   3685
Chromosome Mapping:   2q31-q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.700000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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