PrimePCR™ Probe Assay: DIAPH1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DIAPH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DIAPH1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027087
Assay Design:   Intron-spanning
Chromosome Location:   5:140960367-140961941question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000389054 ENST00000520569 ENST00000518047 ENST00000398562 ENST00000389057 ENST00000398566 ENST00000398557 ENST00000253811 ENST00000546094

Gene Information

This gene is a homolog of the Drosophila diaphanous gene and has been linked to autosomal dominant fully penetrant nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   DIAPH1
Gene Name:   diaphanous homolog 1 (Drosophila)
Aliases:   DFNA1, DIA1, DRF1, FLJ25265, LFHL1, hDIA1
RefSeq:   NC_000005.9 NT_029289.11 NG_011594.1
Ensembl:   ENSG00000131504
Entrez:   1729
Chromosome Mapping:   5q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994400
y-intercept 34.430000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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