PrimePCR™ Probe Assay: RACGAP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RACGAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RACGAP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027960
Assay Design:   Intron-spanning
Chromosome Location:   12:50399113-50400284question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000427314 ENST00000312377 ENST00000454520 ENST00000551016 ENST00000547905 ENST00000552310 ENST00000546595 ENST00000551145 ENST00000548824 ENST00000548644 ENST00000546723 ENST00000552921 ENST00000552157 ENST00000546764 ENST00000551876 ENST00000549777 ENST00000550651 ENST00000434422

Gene Information

The protein encoded by this gene belongs to the GTPase-activating protein (GAP) family. GAPs bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function GAPs negatively regulate Rho-mediated signals. This protein plays a regulatory role in initiation of cytokinesis controlling cell growth and differentiation of hematopoietic cells regulating spermatogenesis and in neuronal proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   RACGAP1
Gene Name:   Rac GTPase activating protein 1
Aliases:   HsCYK-4, ID-GAP, MgcRacGAP
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000161800
Entrez:   29127
Chromosome Mapping:   12q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.630000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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