PrimePCR™ Probe Assay: ACTA2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ACTA2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ACTA2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028813
Assay Design:   Intron-spanning
Chromosome Location:   10:90699374-90701011question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000224784 ENST00000458208 ENST00000544901

Gene Information

The protein encoded by this gene belongs to the actin family of proteins which are highly conserved proteins that play a role in cell motility structure and integrity. Alpha beta and gamma actin isoforms have been identified with alpha actins being a major constituent of the contractile apparatus while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Nov 2008]

Gene Symbol:   ACTA2
Gene Name:   actin, alpha 2, smooth muscle, aorta
Aliases:   AAT6, ACTSA
RefSeq:   NC_000010.10 NG_011541.1 NT_030059.13
Ensembl:   ENSG00000107796
Entrez:   59
Chromosome Mapping:   10q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.830000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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