PrimePCR™ Probe Assay: IKBKG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IKBKG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IKBKG, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028993
Assay Design:   Intron-spanning
Chromosome Location:   X:153780310-153784497question
Amplicon Length:   183
Splice Variants Targeted:   ENST00000440286 ENST00000422680 ENST00000369609 ENST00000369607 ENST00000445622 ENST00000369606 ENST00000413620 ENST00000263518 ENST00000470142 ENST00000393549 ENST00000455588 ENST00000369602 ENST00000369601

Gene Information

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex which activates NF-kappaB resulting in activation of genes involved in inflammation immunity cell survival and other pathways. Mutations in this gene result in incontinentia pigmenti hypohidrotic ectodermal dysplasia and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq Aug 2011]

Gene Symbol:   IKBKG
Gene Name:   inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Aliases:   AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IP, IP1, IP2, IPD2, NEMO
RefSeq:   NC_000023.10 NG_009015.1 NT_167198.1 NG_009896.1
Ensembl:   ENSG00000073009
Entrez:   8517
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.590000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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