PrimePCR™ Probe Assay: GZMB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GZMB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GZMB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029030
Assay Design:   Intron-spanning
Chromosome Location:   14:25102194-25103397question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000415355 ENST00000216341 ENST00000526004 ENST00000382542 ENST00000382540

Gene Information

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize bind and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq Jul 2008]

Gene Symbol:   GZMB
Gene Name:   granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Aliases:   CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100453
Entrez:   3002
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.150000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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