PrimePCR™ Probe Assay: BAMBI, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BAMBI, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BAMBI, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029206
Assay Design:   Intron-spanning
Chromosome Location:   10:28966882-28970262question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000375533 ENST00000542444

Gene Information

This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family whose members play important roles in signal transduction in many developmental and pathological processes. The encoded protein however is a pseudoreceptor lacking an intracellular serine/threonine kinase domain required for signaling. Similar proteins in frog mouse and zebrafish function as negative regulators of TGF-beta which has led to the suggestion that the encoded protein may function to limit the signaling range of the TGF-beta family during early embryogenesis. [provided by RefSeq Jul 2008]

Gene Symbol:   BAMBI
Gene Name:   BMP and activin membrane-bound inhibitor homolog (Xenopus laevis)
Aliases:   NMA
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000095739
Entrez:   25805
Chromosome Mapping:   10p12.3-p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.670000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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