PrimePCR™ Probe Assay: MAP2K6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAP2K6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAP2K6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030567
Assay Design:   Intron-spanning
Chromosome Location:   17:67521068-67522836question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000359094

Gene Information

This gene encodes a member of the dual specificity protein kinase family which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases also known as extracellular signal-regulated kinases (ERKs) act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway this gene is involved in many cellular processes such as stress induced cell cycle arrest transcription activation and apoptosis. [provided by RefSeq Jul 2008]

Gene Symbol:   MAP2K6
Gene Name:   mitogen-activated protein kinase kinase 6
Aliases:   MAPKK6, MEK6, MKK6, PRKMK6, SAPKK3
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000108984
Entrez:   5608
Chromosome Mapping:   17q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.200000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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