PrimePCR™ Probe Assay: TNFRSF10A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TNFRSF10A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TNFRSF10A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031948
Assay Design:   Intron-spanning
Chromosome Location:   8:23058229-23059391question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000221132 ENST00000524158

Gene Information

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL) and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD a death domain containing adaptor protein is required for the apoptosis mediated by this protein. [provided by RefSeq Jul 2008]

Gene Symbol:   TNFRSF10A
Gene Name:   tumor necrosis factor receptor superfamily, member 10a
Aliases:   APO2, CD261, DR4, MGC9365, TRAILR-1, TRAILR1
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000104689
Entrez:   8797
Chromosome Mapping:   8p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.850000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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