PrimePCR™ Probe Assay: TNFRSF9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TNFRSF9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TNFRSF9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0032827
Assay Design:   Intron-spanning
Chromosome Location:   1:7993306-7995156question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000377507

Gene Information

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion survival and development of T cells. It can also induce proliferation in peripheral monocytes enhance T cell apoptosis induced by TCR/CD3 triggered activation and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq Jul 2008]

Gene Symbol:   TNFRSF9
Gene Name:   tumor necrosis factor receptor superfamily, member 9
Aliases:   4-1BB, CD137, CDw137, FLJ43501, ILA, MGC2172
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000049249
Entrez:   3604
Chromosome Mapping:   1p36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 34.650000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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