PrimePCR™ Probe Assay: PPP2R2C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PPP2R2C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPP2R2C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033650
Assay Design:   Intron-spanning
Chromosome Location:   4:6374331-6377622question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000335585 ENST00000506140 ENST00000515571 ENST00000382599 ENST00000507294

Gene Information

The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme which is composed of a catalytic subunit and a constant regulatory subunit that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   PPP2R2C
Gene Name:   protein phosphatase 2, regulatory subunit B, gamma
Aliases:   B55-GAMMA, IMYPNO, IMYPNO1, MGC33570, PR52, PR55G
RefSeq:   NC_000004.11 NT_006051.18
Ensembl:   ENSG00000074211
Entrez:   5522
Chromosome Mapping:   4p16.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.770000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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