PrimePCR™ Probe Assay: BRAF, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BRAF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BRAF, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033856
Assay Design:   Intron-spanning
Chromosome Location:   7:140534615-140549990question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000288602

Gene Information

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway which affects cell division differentiation and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome a disease characterized by heart defects mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers including non-Hodgkin lymphoma colorectal cancer malignant melanoma thyroid carcinoma non-small cell lung carcinoma and adenocarcinoma of lung. A pseudogene which is located on chromosome X has been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   BRAF
Gene Name:   v-raf murine sarcoma viral oncogene homolog B1
Aliases:   B-RAF1, BRAF1, FLJ95109, MGC126806, MGC138284, NS7, RAFB1
RefSeq:   NC_000007.13 NG_007873.1 NT_007914.15
Ensembl:   ENSG00000157764
Entrez:   673
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.020000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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