PrimePCR™ Probe Assay: RABGEF1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RABGEF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RABGEF1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041441
Assay Design:   Intron-spanning
Chromosome Location:   7:66270344-66273928question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000395696 ENST00000442740 ENST00000412398 ENST00000395694 ENST00000447018 ENST00000442104 ENST00000451603 ENST00000451789 ENST00000443156 ENST00000428059 ENST00000456351 ENST00000399150 ENST00000252229 ENST00000377811 ENST00000276062 ENST00000436531 ENST00000436655 ENST00000437651 ENST00000383514 ENST00000380828 ENST00000510829 ENST00000451741 ENST00000450873 ENST00000284957 ENST00000439720 ENST00000437078 ENST00000438954 ENST00000458032 ENST00000400313 ENST00000428416 ENST00000316731

Gene Information

RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5 (RAB5A; MIM 179512) (Horiuchi et al. 1997 [PubMed 9323142]).[supplied by OMIM Mar 2010]

Gene Symbol:   RABGEF1
Gene Name:   RAB guanine nucleotide exchange factor (GEF) 1
Aliases:   FLJ32302, RABEX5, rabex-5
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000154710
Entrez:   27342
Chromosome Mapping:   7q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.910000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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