PrimePCR™ Probe Assay: Gm5506, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Gm5506, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Gm5506, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0033613
Assay Design:   Intron-spanning
Chromosome Location:   18:48045371-48046797question
Amplicon Length:   110
Splice Variants Targeted:   ENSMUST00000076155

Gene Information

This gene may represent an evolving pseudogene of the alpha-enolase (enolase 1 alpha non-neuron) gene which has multiple pseudogenes. This gene has an intact open reading frame as well as strong transcriptional support. The length of encoded protein is conserved compared to the original enolase 1 protein. The exact function of this gene is unknown. [provided by RefSeq Jul 2008]

Gene Symbol:   Gm5506
Gene Name:   Predicted gene 5506
Aliases:   EG433182, Eno1
RefSeq:   NC_000084.6 NT_039674.8
Ensembl:   ENSMUSG00000059040
Entrez:   433182
Chromosome Mapping:   18 C|18

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.980000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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