PrimePCR™ Probe Assay: Bcl2l11, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Bcl2l11, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Bcl2l11, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0024424
Assay Design:   exonic
Chromosome Location:   3:126601736-126601827question
Amplicon Length:   62
Splice Variants Targeted:   ENSRNOT00000041970 ENSRNOT00000033136 ENSRNOT00000000621 ENSRNOT00000067004 ENSRNOT00000000447 ENSRNOT00000007721 ENSRNOT00000016087 ENSRNOT00000044578 ENSRNOT00000051069 ENSRNOT00000022596 ENSRNOT00000054962 ENSRNOT00000027760

Gene Information

proapototic Bcl-2 related protein that interacts with anti-apoptotic Bcl-2 family members to play a role in regulation of apoptosis [RGD, Feb 2006]

Gene Symbol:   Bcl2l11
Gene Name:   Bcl-2-like protein 11
Aliases:   Not Available
RefSeq:   NM_171989 NM_022612 NM_171988
Ensembl:   ENSRNOG00000016551
Entrez:   64547
Chromosome Mapping:   3q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.650000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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