PrimePCR™ Probe Assay: Ptk2b, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Ptk2b, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Ptk2b, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0023883
Assay Design:   intron_spanning
Chromosome Location:   15:48754691-48756883question
Amplicon Length:   71
Splice Variants Targeted:   ENSRNOT00000030007

Gene Information

may phosphorylate focal adhesion kinase FAK; may play a role in actin cytoskeleton reorganization; may mediate thrombin induced astrocyte proliferation [RGD, Feb 2006]

Gene Symbol:   Ptk2b
Gene Name:   Protein-tyrosine kinase 2-beta
Aliases:   Not Available
RefSeq:   NM_017318
Ensembl:   ENSRNOG00000027839
Entrez:   50646
Chromosome Mapping:   15p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.590000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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