PrimePCR™ Probe Assay: MYLK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MYLK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MYLK, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039049
Assay Design:   exonic
Chromosome Location:   3:123333017-123333135question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000360772 ENST00000418370 ENST00000578202 ENST00000346322 ENST00000583087 ENST00000475616 ENST00000360304 ENST00000359169 ENST00000354792

Gene Information

This gene a muscle member of the immunoglobulin gene superfamily encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition using a separate promoter in an intron in the 3' region it encodes telokin a small protein identical in sequence to the C-terminus of myosin light chain kinase that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq Jul 2008]

Gene Symbol:   MYLK
Gene Name:   myosin light chain kinase
Aliases:   DKFZp686I10125, FLJ12216, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK1, smMLCK
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000065534
Entrez:   4638
Chromosome Mapping:   3q21

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