PrimePCR™ Probe Assay: TNF, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TNF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TNF, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040184
Assay Design:   exonic
Chromosome Location:   6:31545204-31545328question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000328965 ENST00000445232 ENST00000594551 ENST00000443707 ENST00000412275 ENST00000449264 ENST00000577810 ENST00000326294 ENST00000448781 ENST00000420425 ENST00000394126 ENST00000356271 ENST00000394128 ENST00000394127 ENST00000422942 ENST00000501516 ENST00000536318 ENST00000431269 ENST00000376122 ENST00000383496 ENST00000264203 ENST00000375144 ENST00000375142 ENST00000401084 ENST00000439554

Gene Information

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation differentiation apoptosis lipid metabolism and coagulation. This cytokine has been implicated in a variety of diseases including autoimmune diseases insulin resistance and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq Jul 2008]

Gene Symbol:   TNF
Gene Name:   tumor necrosis factor
Aliases:   DIF, TNF-alpha, TNFA, TNFSF2
RefSeq:   NC_000006.11 NG_007462.1 NG_012010.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000232810
Entrez:   7124
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.810000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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