PrimePCR™ Probe Assay: ADH5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ADH5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ADH5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040502
Assay Design:   exonic
Chromosome Location:   4:99992995-99993137question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000296412

Gene Information

This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates including ethanol retinol other aliphatic alcohols hydroxysteroids and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde a potent irritant and sensitizing agent that causes lacrymation rhinitis pharyngitis and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq Oct 2008]

Gene Symbol:   ADH5
Gene Name:   alcohol dehydrogenase 5 (class III), chi polypeptide
Aliases:   ADH-3, ADHX, FDH, GSNOR
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000197894
Entrez:   128
Chromosome Mapping:   4q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.150000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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