PrimePCR™ Probe Assay: CYP2B6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CYP2B6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CYP2B6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041698
Assay Design:   exonic
Chromosome Location:   19:41522867-41523086question
Amplicon Length:   190
Splice Variants Targeted:   ENST00000389938 ENST00000449592 ENST00000546474 ENST00000324071 ENST00000593831 ENST00000330446 ENST00000357137 ENST00000360121 ENST00000591344 ENST00000398004 ENST00000412522 ENST00000356290 ENST00000540827 ENST00000428863 ENST00000238831 ENST00000545225 ENST00000254799 ENST00000439371 ENST00000396825 ENST00000378230 ENST00000397762 ENST00000427390 ENST00000560659 ENST00000316397 ENST00000295890 ENST00000299192 ENST00000393276 ENST00000393274 ENST00000377205 ENST00000592502 ENST00000377038 ENST

Gene Information

This gene CYP2B6 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP2B6
Gene Name:   cytochrome P450, family 2, subfamily B, polypeptide 6
Aliases:   CPB6, CYP2B, CYP2B7, CYP2B7P, CYPIIB6, IIB1, P450
RefSeq:   NC_000019.9 NG_000008.7 NG_007929.1 NT_011109.16
Ensembl:   ENSG00000197408
Entrez:   1555
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 37.180000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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