PrimePCR™ Probe Assay: KITLG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KITLG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KITLG, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049380
Assay Design:   exonic
Chromosome Location:   12:88890682-88890814question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000347404 ENST00000228280 ENST00000378535

Gene Information

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development and hematopoiesis all believed to reflect a role in cell migration. In adults it functions pleiotropically while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   KITLG
Gene Name:   KIT ligand
Aliases:   DKFZp686F2250, FPH2, KL-1, Kitl, MGF, SCF, SF, SHEP7
RefSeq:   NC_000012.11 NG_012098.1 NT_029419.12
Ensembl:   ENSG00000049130
Entrez:   4254
Chromosome Mapping:   12q22

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