PrimePCR™ Probe Assay: TBXAS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TBXAS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TBXAS1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049437
Assay Design:   exonic
Chromosome Location:   7:139529257-139572122question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000425687 ENST00000438104 ENST00000336425 ENST00000448866 ENST00000455353 ENST00000458722 ENST00000411653 ENST00000263552 ENST00000416849 ENST00000436047 ENST00000414508 ENST00000539806

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. However this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to thromboxane A2 a potent vasoconstrictor and inducer of platelet aggregation. The enzyme plays a role in several pathophysiological processes including hemostasis cardiovascular disease and stroke. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2008]

Gene Symbol:   TBXAS1
Gene Name:   thromboxane A synthase 1 (platelet)
Aliases:   CYP5, CYP5A1, FLJ52771, GHOSAL, THAS, TS, TXAS, TXS
RefSeq:   NC_000007.13 NG_008422.1 NT_007914.15
Ensembl:   ENSG00000059377
Entrez:   6916
Chromosome Mapping:   7q34-q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.840000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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