PrimePCR™ Probe Assay: PTGS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PTGS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PTGS1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049942
Assay Design:   exonic
Chromosome Location:   9:125154551-125154680question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000223423 ENST00000362012 ENST00000540753 ENST00000373698

Gene Information

Prostaglandin-endoperoxide synthase (PTGS) also known as cyclooxygenase is the key enzyme in prostaglandin biosynthesis and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2 which differ in their regulation of expression and tissue distribution. This gene encodes PTGS1 which regulates angiogenesis in endothelial cells and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis. Alternative splicing of this gene generates two transcript variants. The expression of these two transcripts is differentially regulated by relevant cytokines and growth factors. [provided by RefSeq Jul 2008]

Gene Symbol:   PTGS1
Gene Name:   prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
Aliases:   COX1, COX3, PCOX1, PGG/HS, PGHS-1, PGHS1, PHS1, PTGHS
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000095303
Entrez:   5742
Chromosome Mapping:   9q32-q33.3

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